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Description
In cancer cells, pyruvate is continually converted to lactate in the cytoplasm, thus hampering glucose entry into mitochondria
Visual inspection was conducted for each spectrum, excluding poorly fitted spectra from analysis
After aspiration and washing, 100 l of substrate solution was added to each well and incubated for 30 min at room temperature. Then, 100 L of a stop solution was added, and color changes were measured at 450 nm using a BioTek ELISA reader
Therefore, a decrease in intestinal dopamine levels is closely related to the development of IBD
