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The tissue was thawed in Type I water at room temperature, and excess connective tissue was manually removed

The first use may be considered as the isolation of -amylase by using an insoluble substrate, starch, in 1910 just three years after the discovery of chromatography by Tsewett [6,9]

Results Using 53 search terms related to digital health and the ten selected rare diseases (see Supplementary Table 1), a total of 8373 clinical trial records and publications were initially retrieved from Clinicaltrials.gov, the ICTRP, Cochrane, PubMed and Web of Science

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