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Based on the here-presented biochemical, structural and computational evidence, we propose that arGSTs utilize a GSH-dependent Brnsted base mechanism for conversion of the semi-stable LDOX product flavan-3,3,4-triol ( 4 ) or flavan-3-on-4-ol ( 7 ) into flav-2-en-3,4-diol ( 8 -B4), the 4-hydration species of cyanidin ( 8 ) (Extended Data Fig

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The slides were then read using the InnoScan 710-IR slide scanner (Innopsys) at an excitation wavelength of 785 nm for IRDye800CW and an excitation wavelength of 670 nm for Fast Green FCF