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2008, 11(4): 687-692 Link to Abstract Let us help

A volume of 4-NPG (final concentration of 1 mM) was added to the reaction and decrease in absorbance at 305 nm was recorded on an Envision multilabel plate reader (Perkin Elmer)

Furthermore, increased copper levels can increase reactive oxygen species (ROS) production and activate the NF-B signalling pathway, exacerbating inflammatory changes within the vascular wall and promoting atherosclerosis (25)

Nat Genet 49:13851391
