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Description
The supernatant was discarded and the beads were washed thrice with LSB-2 containing 150 mM NaCl which is termed LSB-3 (10 mM Tris pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 150 mM NaCl, and 1 mM PMSF) with or without IGEPAL CA-630 followed by incubation at 4 C/1 h
Mice were allowed one additional week for recovery
178.5% vs 129.3%
In-vitro fibroblast studies indicate that GHK-Cu upregulates transcription of collagen and elastin genes and supplies copper as a cofactor for lysyl oxidase, the enzyme that cross-links these matrix proteins
