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Actin was the protein loading control

Supernatants containing lentiviral particles were collected and replaced with fresh medium daily for 3 consecutive days
Gene expression and the nervous system Broad Institute Connectivity Map data reveals that GHK influences the expression of a staggering number of human genes, with 50% or greater change in expression in roughly 31% of all human genes examined

doi: 10.1152/japplphysiol.00925.2012
