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The frozen tissue or cell samples were mixed with 3.6% perchloric acid (v/v, prepared in distilled water [318-90105, Nippon Gene]) (prepared using 0.2 and 0.3 mL of 3.6% PCA per cell sample [1 10 6 and 4 10 6 cells for 293 and HAP1 wild-type/ XPR1 KO cells] and 100 mg tissue samples, respectively)
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The conversion factors for converting IU to mg of alpha-tocopherol are: 0.67 for RRR-alpha-tocopherol (and its esters) 0.45 for all-rac-alpha-tocopherol (and its esters) Here are some example calculations showing how to use these conversion factors: Example 1
GLP-1 is an important, gut-derived, glucose homeostasis regulator that is released after the oral ingestion of carbohydrates or fats
