gst-protein complexes be removed from glutathione beads GST-Tag Problem with GST-fusion protein elution-
Description
doi: 10.1080/10408398.2018.1474170 [DOI] [PubMed] [Google Scholar] 106.Yuan Z, Liu C, Sun Y, et al
Cronin, S
The staining solution, containing 5 ml DMEM, 8 l FDA (5 mg/ml) and 50 l PI (2 mg/ml) was added to the wells, cells were incubated at room temperature for 4-5 minutes in the dark, washed with PBS and examined on Eclipse Ti-E confocal laser scanning microscope (CLSM) with A1 confocal module (Nikon Corporation, Japan) and CFI Plan Apo VC 20/0.75 objective lens with a life support system, maintaining normal culturing conditions (37C, 5% CO 2 )
(2021) 5:e2100115
