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The results of a sequential supplementation trial in 53 men and women indicated that after optimization of folate status with folic acid supplements, vitamin B 12 became the major determinant of plasma homocysteine levels (30)

established a modified DTT technique that requires lower DTT concentrations, is less time-consuming, and appears to avoid denaturation of Kell antigen [52]

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The neurological consequences of untreated B12 deficiency can be serious and potentially permanent, including subacute combined degeneration of the spinal cord, which affects both sensory and motor pathways