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2000;16(Suppl 1):s17-20

After 24 h, bilirubin (0.1 M) was added into the cells, then after 6 h, the cells were treated with or without doxorubicin (1 M) for 24 h, collected the cells and washed with cold PBS and then resuspend cells in 1 Binding Buffer, and then transfer 100 l of the solution (1 10 5 cells) to a 2 ml culture tube, and add 5 l of FITC Annexin V and 5 l PI, and gently vortex the cells and incubate for 15 min at 25C in the dark room, and finally add 400 l of Binding Buffer to each tube, and then analyze it by flow cytometry

Recommended intake S-Acetyl-Glutathione Capsules: As a dietary supplement, take one capsule twice daily with plenty of water
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