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Those on the HFD received daily gavage administration of CB-839 (200 mg kg 1 body weight) or vehicle for 19 days (25% (w/v) hydroxypropyl--cyclodextrin (ThermoFisher) in 10 mmol l 1 citrate buffer (pH 2)), while the standard chow group received vehicle only

Multiply that volume by 100 to convert to syringe units on a U-100 insulin syringe

When glutathione neutralizes a free radical, it becomes "oxidized." In this state, it is spent and cannot protect the cell anymore

For monotherapy details, see the tirzepatide protocol page