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RPMI 1640 (GIBCO) supplemented with 10% (v/v) FBS (Sigma), 20 ng/ml GM-CSF (Sigma), 1% (v/v) Pen-strep (Sigma), and 1% (v/v) sodium pyruvate (GIBCO) was used to culture the cells
This observation is supported by clinical studies showing that chronic stress and dysregulation of the stress response were found to contribute to the maintenance and propagation of various CSCs in patients suffering from chronic stress, anxiety, depression, and suicidal behaviour [226, 251, 252]
Decreased neurogenesis after cholinergic forebrain lesion in the adult rat
