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Ultrathin sections (80 nm thick) were cut with an ultramicrotome (Leica, EMUC6, Germany), and then stained with uranyl acetate and lead citrate and finally examined by a Tecnai G 2 Spirit TWIN transmission electron microscope (FEI, Hillsboro, OR, USA)

Gene sequencing technology has revealed that the hub genes HIF1A, HILPDA, and SCD are closely associated with ferroptosis, T2DM, hypoxia, and lipid metabolism [69]
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