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[DOI] [PMC free article] [PubMed] [Google Scholar] 112.Cohen J L, Mariwall K
Hyperketonemia increases tumor necrosis factor-alpha secretion in cultured U937 monocytes and type 1 diabetic patients and is apparently mediated by oxidative stress and cAMP deficiency
Incubate at room temperature for 15 minutes
Cells were then infected with GFP control, CHAC1, or MitoCHAC1 lentivirus and then selected with 100 g/mL hygromycin (Invivogen, ant-hg-1) for 5d prior to use for experimentation
