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No fructose, sucrose, or artificial sweeteners
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The wells were excited at a wavelength of 550 nm and emission was detected at 610 nm (readings taken every 5 min)

Binding data obtained using this method have previously been validated by direct comparison to results of nuclear magnetic resonance, circular dichroism, capillary electrophoresis, and other physicochemical methods (e.g., [51,87,88,89,90])
