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Therefore, the subsequent PI3K/Akt impairment suppresses this vital Nrf2-mediated antioxidant defenses while concurrently activating NFB-driven inflammation, thereby exacerbating mitochondrial ROS production and cytokine release (e.g., IL-6, TNF- , IL-1) in diabetic complications (41, 44)

Double mutants between tph-1 and tbh-1 resembled tph-1 mutants on all diets, pointing to a central role of serotonin across conditions, and a secondary role for octopamine

Regularly monitoring key biomarkers is non-negotiable
