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The concentration of inducible nitric oxide synthase (iNOS), total nitric oxide synthase (tNOS), hydrogen peroxide (H 2 O 2 ), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and superoxide dismutase (SOD) and so on in the intestinal mucosae were determined by using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)

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A common process condition optimization method, Box-Behnken design, was used to optimize the operating variables such as temperature, pH, and enzyme quantity [34]

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