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Fahrenholtz CD, Beltran PJ, Burnstein KL

Variation in metabolic regulation in LB versus BSF reflects the highly adaptable nature of PAO1 metabolism with respect to their nutritional environment and suggests that metabolomics results obtained in different growth media are not necessarily transferrable

For NADPH-dependent GSSG reduction assays, 50 g of liver lysate in 10 l was mixed with 190 l of reaction mixture containing 1 mM GSSG (Sigma-Aldrich G4626) and 200 M NADPH (Sigma-Aldrich N7505) in 100 mM potassium phosphate, pH 7.0, 2 mM EDTA 10,23,41

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