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Description
Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)
Contamination is cumulative
Hyperthyroidism causes hair to thin, while hypothyroidism leads to coarse hair in odd places
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