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The dosing schedule and repeated injection protocol were designed in accordance with manufacturer guidelines and supported by earlier published studies ( Figure 1 2.4 Schedule of sample collection and processing of the sample The calving date was estimated using the farm/stock register, with a margin of one week before or after the anticipated calving date, as recorded by the Institutes Livestock Farm

For regulatory agencies to ultimately modify their recommendations from minimal amounts of nutrients to stave off diseases of nutritional deficiency to optimal amounts of nutrients to promote health, significant investment and novel experimental approaches in the nutritional sciences must occur