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Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media
GSH is decomposed into a gamma-glutamyl amino acid through gamma-glutamyl transpeptidase
Mateos MV, Cavo M, Blade J, et al
