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S2 was ultracentrifuged at 165,000 g for 2 h at 4 C, generating supernatant S3 (cytosolic proteins) and pellet P3 enriched in light intracellular membranes (LM)

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They demonstrated that miR-365-3p/ETS homologous factor (EHF, a KRT16transcription factor) axis could reduce OSCC cell migration, invasion, metastasis, and chemoresistance to 5-fluorouracil through the suppression of KRT16