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Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)

Injury recovery Investigated in animal studies on recovery from soft-tissue injury, including tendon, ligament, and muscle models

demonstrated that supplementation with NAC in animals undergoing oxidative stress from ethanol infusions led to decreased inflammatory markers such as aspartate aminotransferase (AST), alanine transaminase (ALT), and GGT dramatically [39]

Relevantly, the extract favorably ameliorated CVD risk factors by causing a significant decrease in total cholesterol, LDL-C, and atherogenic index (Siddiqi et al., 2012)