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The plate was then washed three times with phosphate buffer before the addition of 100 l chemiluminescent substrate following manufacturers instructions (Chemiluminescence ELISA Substrate, Roche 11582950001), and the plate immediately analyzed with a microplate reader (FLUOstar OMEGA, BMG Labtech)
Every step of our process is personalized and designed for optimal results: We begin by hearing your full story, your activity history, pain patterns, lifestyle, and goals
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