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3.3 FGF2 downregulates reactive oxygen species in 5% O 2, increasing redox-sensitive PDH activity We previously reported that culturing hPSCs at 5% O 2 reduces levels of ROSkey second messengers known to modulate FGF2 signalingand that FGF2 signaling likely contributes to ROS suppression in 5% O 2 , as MEK1/2 inhibition led to increased ROS levels under 5% but not 21% O 2 ( 2 (Figure 3A)
Mucins are encoded by 17 MUC genes [35, 36] and once transcribed, they are translated and stored as granules in the goblet cells before being secreted

of Molecular Embryology, Albert-Ludwigs-University Freiburg for preparing figures for this review

Cage bedding was changed every other day with new sterile bedding throughout the entire experiment
