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In fact, plant PCD research is still in its infancy, with progress marked by sporadicreports and isolated studies

After the cross-linking reaction, 10 L of the reaction mixture was suspended into an equal volume of sample buffer for SDS-PAGE (Nacalai-Tesque) analysis, incubated for 3 min at 100 C, loaded onto a 12% SDS-PAGE gel, and the gel was stained by CBB stain One Super (Nacalai-Tesque)

Used it recently on a demonstration
