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Discussions focused on the feasibility of clinical trial site accreditation, based on other models in the health care industry
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The cells were treated with capsaicin followed by 72 h of incubation and treatment was repeated for a further 6 days
After changing culture medium to DMEM without serum, siRNAs (12.5 nM final concentration) were simultaneously transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Inc.) and incubated at 37C for 24 h
