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The FFA-induced rise in intracellular Ca 2+ is substantially reduced when cells are cultured in a Ca 2+ free medium, and is abolished upon treatment of cells with the Ca 2+ channel inhibitor nicardipine [292] or when using BSA (which binds fatty acids) [251]
The high cost of raw materials, complex synthetic procedures, and the use of specialized reagents contribute significantly to overall production costs
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Another mechanism that might better explain the finding of residual gastric content under these conditions (illustrated in Fig
