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Then the potential biomarkers were identified by comparing precise mass-to-charger ratio (M/z), secondary fragments, and isotopic distribution with the standards from Human Metabolome Database (HMDB) database, Lipidmaps (v2.3), METLIN database and databases containing more than 4000 metabolite information self-built by Shanghai Lu-Ming Biotech Co., Ltd

Using proper techniqueincluding allowing the alcohol to dry completely before injectionmay also reduce reaction frequency