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Bacteria were harvested by centrifugation, resuspended in Buffer Sol-ER (50 mM HEPES pH 7.9, 180 mM NaCl, 5 mM KCl, 1 mM EDTA, 5% Glycerol, 0.05% Triton, 1 mM DTT) plus 1 mM PMSF and lysed by sonication after 1 mg/mL lysozyme treatment

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Malnutrition Some patients experience appetite suppression to the point of nutrient deficiency, which can affect gum health and healing, said Austin