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Description
Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min

This dosing regimen was selected based on prior studies demonstrating effective GCLC activation and antioxidant enhancement under oxidative stress conditions

J., Zeng, Y

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