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To maintain a low temperature during homogenization, the tube was placed in a beaker of ice and processed with a homogenizer at 50 rpm for two minutes
While Wnt7a has a limited role in impaired dendritic arborization of dentate granule neurons, Wnt7b, which is also expressed in the hippocampus, increases dendritic arborization in hippocampal neurons and accelerates dendritic development by increasing neurite length and branching [61] by Wnt/-catenin signaling [62]
[6] [7] It is found in both raw and cooked beef, together with other cobalamins

Markart P, Korfhagen TR, Weaver TE, Akinbi HT: Mouse lysozyme M is important in pulmonary host defense against Klebsiella pneumoniae infection