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Description
The solutions were mixed and incubated at 25C for about 5 min

Rodrigues DB, Mariutti LRB, Mercadante AZ (2016) An in vitro digestion method adapted for carotenoids and carotenoid esters: moving forward towards standardization
It uses needle insertion without incision or tissue removal and carries a zero-day global period

Acta Neurobiol Exp (Wars) 2010